Tegretol Carbamazepine

posted on 27 Aug 2012 07:46 by tegretolcarbamaade
Tegretol Carbamazepine

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Serum, ml Transfer to next tube, ml . . Antigen, ml Reciprocal of final serum Tube No. 0.8 0.2 0.5 0.5 0.5 0.5 0.5 dilution ! 10 20 0.5 0.5 0.5 0.5 0.5|0.5 6 ! 7 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 40 I 80 ! 160 320 I 640 \ 1280 Tegretol Carbamazepine I 2560 I i I Antigen control 0.5 0.5 * Discard 0.5 ml. pipetted. These are mixed by taking a clean pipet and alternately sucking up about 0.5 ml into the pipet and blowing it back into the tube several times. Following this, 0.5 ml of the 1:5 dilution in tube i is transferred into tube 2. This process of mixing and then transferring 0.5 ml Tegretol Carbamazepine into the next tube is followed with each Tegretol Carbamazepine tube in the series, using a clean pipet for each transfer. When the last tube is reached, 0.5 ml is discarded. An additional tube containing only saline solution is included for a control on the antigen suspension. Next, 0.5 ml of antigen suspen- sion is placed in each tube, and the tubes mixed by shaking. The test is placed in a constant-temperature water bath for 2-4 hr. The tempera- ture at which the water bath is set may be 37-55C, or any constant tem- perature within this range, depending on the system being studied. As a rule, the Tegretol Carbamazepine arbitrary selection of 37 will yield excellent results with bac- terial antigens. The clumping may be read after this preliminary incuba- tion, but as a general rule, more pronounced reactions are secured follow- ing storage in the refrigerator for 12-18 hr. Clumping may also be accelerated by centrifuging the tubes. When a tube agglutination is read, each tube starting with the antigen control is tapped 4-5 times with the finger tip until the cells are resus- pended. The antigen control should display uniform turbidity in con- trast to the clumping seen in a positive reaction. The size of the clumps will depend on the potency of the antiserum and the physical nature of the antigen. Cells like the ''O" antigen suspensions of Salmonella usu-
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posted on 26 Aug 2012 07:46 by tegretolcarbamaade

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